home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9409a.zip
/
M9490153.TXT
< prev
next >
Wrap
Text File
|
1994-09-03
|
3KB
|
42 lines
Document 0153
DOCN M9490153
TI Physiological effects of HIV infection on human intestinal epithelial
cells: an in vitro model for HIV enteropathy.
DT 9411
AU Asmuth DM; Hammer SM; Wanke CA; Division of Infectious Diseases, New
England Deaconess Hospital,; Boston, MA 02215.
SO AIDS. 1994 Feb;8(2):205-11. Unique Identifier : AIDSLINE MED/94318204
AB OBJECTIVES: To determine the role of direct infection of intestinal
cells with HIV-1 in the pathogenesis of HIV-related enteropathy.
METHODS: We infected HT-29-18-C1 intestinal cells with the IIIB strain
of HIV and examined the physiologic effects of enterocyte function.
Dipeptidase-IV, aminopeptidase-N, gamma glutamic transferase, and
alkaline phosphatase were measured in HIV-infected and control cultures.
The cellular second messengers intracellular calcium and cyclic
adenosine monophosphate were also measured in infected and control
cultures. RESULTS: A persistent infection was established for > 95 days
with peak supernatant reverse transcriptase and HIV p24 antigen levels
of 5.17 log10 c.p.m./ml and 45 ng/ml, respectively. Brush-border enzyme
activity (nmol of product/min/mg protein) tended to be lower in infected
cell cultures compared with controls early in infection (P < 0.02).
Baseline second messenger concentrations were similar but infected
cultures responded to stimulation with a calcium ionophore with an
exaggerated increase in intracellular calcium (P = 0.03). CONCLUSIONS:
These results suggest that absorptive and secretory function of
enterocytes may be altered by direct HIV infection and that additional
physiologic experiments with this in vitro model may lead to a better
understanding of the clinical syndrome of HIV enteropathy.
DE Alkaline Phosphatase/METABOLISM Aminopeptidases/METABOLISM Antigens,
CD4/BIOSYNTHESIS Calcium/METABOLISM Carcinoma/PATHOLOGY Colonic
Neoplasms/PATHOLOGY Cyclic AMP/METABOLISM Dipeptidases/METABOLISM
Gamma-Glutamyltransferase/METABOLISM Gastrointestinal
Diseases/COMPLICATIONS/MICROBIOLOGY Human HIV Infections/COMPLICATIONS
HIV-1/*PHYSIOLOGY/PATHOGENICITY Intestinal
Mucosa/*MICROBIOLOGY/ULTRASTRUCTURE Microvilli/ENZYMOLOGY Second
Messenger Systems Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't,
P.H.S. Tumor Cells, Cultured JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).